Louis G. Chicoine, MD

Louis G. Chicoine, MD, is a member of the Section of Neonatology at Nationwide Children’s Hospital and an Assistant Professor of Pediatrics at The Ohio State University College of Medicine. As a principal investigator in the Center for Gene Therapy at The Research Institute at Nationwide Children’s Hospital, Dr. Chicoine’s research interests are focused on the study of normal and abnormal lung and lung vascular development. Several ongoing studies using models ranging from cell culture to isolated lung, and techniques ranging from siRNA interference to physiological studies are directed at answering questions relating to NOS expression and NO production, and their affect on pulmonary artery tone during development. Gene therapy using NOS directed at the lung might be expected to decrease pulmonary vascular resistance and arrest pulmonary vascular remodeling associated with PPHN, and be more efficacious than inhaled NO therapy. Dr. Chicoine is currently utilizing a rat model of PPHN to study the effects and potential toxicities of gene therapy with NOS. These studies are the first step in providing more effective and safer treatment to babies born with PPHN cared for in the Neonatal Intensive Care Unit.

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Gender:

• Male

Languages Spoken:

• English

Research Center:

• Center for Gene Therapy

Areas of Interest:

• Our lab is focused on the study of normal and abnormal lung and lung vascular development. Successful transition from the high resistance fetal pulmonary circulation to the low resistance postnatal pulmonary circulation involves a shift in the balance between vasoconstriction and vasodilation. Pulmonary endothelial nitric oxide synthase (eNOS) is an important source of the endogenous vasodilator nitric oxide (NO). Changes in eNOS may activity be an important contributor to the decrease in pulmonary vascular resistance normally seen after birth. Several ongoing studies using models ranging from cell culture to isolated lung, and techniques ranging from RNA interference to physiological studies are directed at answering questions relating eNOS expression and NO production, and their affect on pulmonary artery tone during development. Under certain conditions this normal progression from the high resistance pulmonary arteries of the fetus to the low resistance pulmonary arteries of the neonate fails, causing persistent pulmonary hypertension of the newborn (PPHN). PPHN continues to have high morbidity and mortality despite advances in neonatal intensive care. The hallmark of PPHN is abnormally constricted and thickened pulmonary arteries. Several poorly understood mechanisms are likely responsible for these changes that include smooth muscle cell migration, proliferation, and hypertrophy. Current treatment modalities are often of limited value and are associated with high degrees of morbidity. With its anti-mitogenic and vasodilatory properties, alterations in pulmonary NO production are key in the pathogenesis of PPHN. Clinical trials using inhaled NO for the treatment of PPHN demonstrated limited efficacy with a failure rate of between 40 and 50%. Gene therapy using inducible NO synthase (iNOS) directed at the lung might be expected to decrease pulmonary vascular resistance and arrest pulmonary vascular remodeling associated with PPHN, and be safer and more efficacious than inhaled NO therapy. Therefore, we are currently utilizing a neonatal rat model of pulmonary hypertension to study the effects of gene therapy with iNOS on pulmonary vascular reactivity and remodeling, as well as any potential toxicities of iNOS gene therapy. These studies are the first step in providing more effective and safer treatment to babies born with PPHN cared for in the neonatal intensive care unit.

Research Funding*:

• Diverse Strategies to Correct the Dystrophin Gene Using Vascular Delivery, National Institutes of Health
• Cationic amino acid transporters and lung NO production, National Institutes of Health, HLBI
• Pulmonary Vascular Targeting of Gene Delivery Using Directed Evolution of AAV, National Heart, Lung and Blood Institute
• The Role of Endogenous Nitric Oxide Synthase Inhibitors in Neonatal Pulmonary Hypertension, The Research Institute at Nationwide Children’s Hospital/OSU

Medical School

• University of Texas
  Date Completed: 05/26/1990

Internship

• Stanford University
  Date Completed: 06/30/1991

Residency

• Stanford University
  Date Completed: 06/30/1993

Fellowship

• University of Colorado Health Sciences Center
  Date Completed: 06/30/1996

Department:

• Pediatrics

Section:

• Neonatology

Speciality:

• Neonatal-Perinatal Medicine, Certified
  
• Pediatrics
  

Date of Appointment at Children's Hospital:

• 03/26/2004

• Hongmei Cui, Bernadette Chen, Louis G Chicone, Leif D Nelin. 2011. Overexpression of cationic amino acid transporter-1 increases nitric oxide production in hypoxic human pulmonary microvascular endothelial cells.  Clin Exp Pharmacol Physiol. no. Clin Exp Pharmacol Physiol. 2011 Sep 16. doi: 10.1111/j.1440-1681.2011.05609.x. [Epub ahead of print]PMID:21923750. (September) (IF: 1.96)
• Bevan AK, Duque S, Foust KD, Morales PR, Braun L, Schmelzer L, Chan CM, McCrate M, Chicoine LG, Coley BD, Porensky PN, Kolb SJ, Mendell JR, Burges AH, Kaspar BK. 2011. Systemic Gene Delivery in Large Species for Targeting Spinal Cord, Brain, and Peripheral Tissues for Pediatric Disorders.  Molecular Therapy. no. Mol Ther. 2011 Aug 2. doi: 10.1038/mt.2011.157. [Epub ahead of print]. (August) (IF: 7.149)
• Cua CL, Rogers LK, Chicoine LG, Augustine M, Jin Y, Nash PL, Nelin LD. 2011. Down Syndrome patients with pulmonary hypertension have elevated plasma levels of asymmetric dimethylarginine.  Eur J Pediatrics. Vol. 170, no. 7. (July): 859-863. (IF: 1.644)
• Louis Chicoine, Michael Stenger, Hongmei Cui, Andrea Calvert, Rebecca Evans, Boyce English, Yusen Liu, and Leif Nelin. 2011. Nitric oxide suppression of cellular proliferation depends on cationic amino acid transporter activity in cytokine stimulated pulmonary endothelial cells.  American Journal of Physiology - Lung Cellular and Molecular Physiology. Vol. 300, no. 4. (April): L596-604. (IF: 4.137)
• Rodino-Klapac LR, Montgomery CL, Mendell JR, Chicoine LG. 2011. AAV-Mediated gene therapy to the isolated limb in rhesus macaques.  Methods Mol Biol. Vol. 709. : 287-298. (IF: 13.9)
• Louise R Rodino-Klapac, Chrystal L Montgomery, Jerry R Mendell, Louis G Chicoine. 2011. AAV Mediated Gene Therapy to the Isolated Limb in Rhesus Macaques. In Muscle Gene Therapy-Methods Mol Biol. Edited by Dongsheng Duan. Columbus, Ohio: Humana Press. (Formally Accepted)
• Preston, Thomas J. BS CCP, Ratliff, Todd M.AS RN, Gomez, Daniel BS CCP,Olshove, Vincent F. Jr.BS CCP, FPP, Nicol, Kathleen K. MD, Sargel, Cheryl L. PharmD, Chicoine, Louis G. MD. 2010. Modified surface coatings and their effect on drug adsorption within the extracorporeal life support circuit.  J Extra Corpor Technol. Vol. 42, no. 3. (September): 199-202.
• Stenger MR, Rose MJ, Joshi MS, Rogers LK, Chicoine LG, Bauer JA, Nelin LD. 2010. Inhaled nitric oxide prevents 3-nitrotyrosine formation in the lungs of neonatal mice exposed to >95% oxygen.  Lung. Vol. 188, no. 3. (June): 217-227. (IF: 1.734)
• Yi Jin, Thomas Calvert, Bernadette Chen, Louis Chicoine, Mandar Joshi, John Bauer, Yusen Liu, and Leif Nelin. 2010. Mice deficient in Mkp-1 develop more severe pulmonary hypertension and greater lung protein levels of arginase in response to chronic hypoxia.  Am J Physiol Heart Circ Physiol.. Vol. 298, no. 5. (May): H1518-1528. (IF: 3.88)
• Inimary T Toby, Louis G Chicoine, Hongmei Cui, Bernadette Chen, Leif D Nelin. H. 2010. Hypoxia-induced proliferation of human pulmonary microvascular endothelial cells depends on epidermal growth factor receptor tyrosine kinase activation.  Am J Physiol Lung Cell Mol Physiol.. Vol. 298, no. 4. (April): L600-606. (IF: 4.137)
• Louise R. Rodino-Klapac, Chrystal L Montgomery, William G Bremer, Vinod Malik, Kimberly M Shontz, Nancy Davis, Spencer Sprinkle, Katherine J Campbell, Zarife Sahenk, K Reed Clark, Chirstopher M Walker, Jerry R Mendell, Louis G Chicoine. 2010. Persistent expression of FLAG-tagged micro dystrophin in nonhuman primates following intramuscular and vascular delivery.  Molecular Therapy. Vol. 18, no. 1. (January): 109-117. (IF: 7.149)
• Chicoine LG, Stewart JA Jr, Lucchesi PA. 2009. Is Resveratrol the Magic Bullet for Pulmonary Hypertension?.  Hypertension. Vol. 54, no. 3. (September): 473-474. Hypertension. 2009 Sep;54(3):473-4. Epub 2009 Jul 13.. (IF: 6.908)
• Martin PT, Xu R, Rodino-Klapac LR, Oglesbay E, Camboni M, Montgomery CL, Shontz K, Chicoine L, Clark KR, Sahenk Z, Mendell JR, Janssen PM. 2009. Overexpression of Galgt2 in skeletal muscle prevents injury resulting from eccentric contractions in both mdx and wild-type mice.  Am J Physiol Cell Physiol.. Vol. 296, no. 3. (March): C476-488. (IF: 3.817)
• Elizabeth Kathleen Natale, Jessica L Slutzky, Louis G Chicoine. 2009. Resveratrol reverses both thomboxanne A2 receptor and non receptor (k+) mediated pulmonary vasoconstriction [Abstract]. FASEB Journal (Published)
• Louis G Chicoine, Mark Girton, Adam S Feuer, Leif Nelin. 2009. MKP-1 Deficiency Augments LPS-Induced NO Production in the Lung [Abstract]. Pediatric Academic Society - May 2008 [Peer Reviewed] (Published)
• Gomez D, Preston T, Olshove V, Chicoine L, Nicol K. 2009. Air Handling Capabilities of Common ECLS Components. [Abstract]. 24th Annual CNMC Symposium, Keystone, Colorado [Peer Reviewed] (Published)
• Louis G Chicoine, Mark R Girton, Adam S Feuer, Leif D Nelin. 2009. Mice Deficient in MKP-1 Develop Severe Pulmonary Hypertension in Hypoxia. [Abstract]. Pediatric Academic Society Annual Meeting May 2008 [Peer Reviewed] (Published)
• Jessica Lauren Slutzky, Elizabeth K Natale, Spencer L Sprinkle, Louis G Chicoine. 2009. Resveratrol prevents RV/T changes in neonatal model of hypoxia induced pulmonary hypertension. [Abstract]. FASEB Journal (Published)
• Zhaosheng Han, Arthur J Pope, Arturo J Cardounel, Leif D Nelin, Louis G Chicoine. 2009. Increased ADMA/protein methylation modulates nitric oxide generation in hypoxic human lung microvascualr endothelial cells. [Abstract]. FASEB Journal (Published)
• Thomas J Calvert, Louis G Chicoine, Yusen Liu, Leif D Nelin. 2009. Inhibition of L-arginine uptake prevents endotoxin-induced hypotension in mitogen activated protein kinase phosphatase-1 Deficient Mice [Abstract]. Pediatric Academic Societies' Annual Meeting [Peer Reviewed] (Published)
• Cua Clifford L, Chicoine LG, Nelin LD, and Mullen Mary. 2009. Pulmonary Hypertension in the Down Syndrome Population. In Cardiology Research and Clinical Developments Series. New York, New York: Nova Science Publishers. 327.
• Stein MA, Preston TJ, Chicoine LG, Shepherd ES, Nankervis CA, Nelin LD. 2009. Randomized Controlled Trial Comparing Fluidic Flip Nasal CPAP to SiPAP in the NICU (Poster Presentation) [Abstract]. Pediatric Academic Societies' Annual Meeting [Peer Reviewed] (Published)
• Chang R, Chicoine LG, Cui H, Kanagy NL, Walker BR, Liu Y, English BK, Nelin LD. 2008. Cytokine-induced arginase activity in pulmonary endothelial cells is dependent on Src family tyrosine kinase activity.  Am J Physiol Lung Cell Mol Physiol.. Vol. 295, no. 4. (October): L688-L697. (IF: 4.137)
• Calvert TJ, Chicoine LG, Liu Y, Nelin LD. 2008. Deficiency of mitogen-activated protein kinase phosphatase-1 results in iNOS-mediated hypotension in response to low-dose endotoxin.  Am J Physiol Heart Circ Physiol.. Vol. 294, no. 4. (April): H1621-H1629. (IF: 3.88)
• Olshove VF, Preston TJ, Gomez D, Burnside JB, Chicoine LG, Nicol KK. 2008. Assessment of a platelet bolus on an in-vitro extracorporeal life support (ECLS) circuit [Poster Presentation] [Abstract]. 24th Annual CNMC Symposium, Keystone, Colorado. (February) [Peer Reviewed] (Published)
• Inimary T. Toby, Louis G Chicoine, Hongmei Cui, Yusen Liu, Leif D Nelin. 2008. Hypoxiainduced Profiferation of Human Pulmonary Microvascular Endothelial Cells Depends on Epidermal Growth Factor Receptor Tyrosine Kinase Activity [Abstract]. Pediatric Academic Societies' Annual Meeting [Peer Reviewed] (Published)
• Hongmei Cui, Louis G Chicone, Yusen Liu, Leif D Nelin. 2008. The Cytotoxic Efforts of Inducible Nitric Oxide Synthase Depend on L-1rginine Uptake in Pulmonary Arterial Endothelial Cells [Abstract]. Pediatric Academic Societies' Annual Meeting [Peer Reviewed] (Published)
• Inimary T Toby, Louis G Chicoine, hongmei Cui, and Leif D Nelin. 2008. Hypoxia-induced profiferation of human microvascular endothelial cells depends on epidermal growth factor receptor (EGFR) tyrosine kinase activity [Abstract]. FASEB Journal (Published)
• Peter Richardson, Melanie Stein, Stephen E Welty, Louis G Chicoine, Alfred l Gest, Leif D Nelin. 2008. The Effect of PEEP or CPAP on Thoraco-Abdominal Asynchrony in Neonatal Patients with BPD [Abstract]. Pediatric Academic Societies' Annual Meeting [Peer Reviewed] (Published)
• Clifford L Cua, Lynette K Rodgers, Molly Augustine, Yi Jin, Louis G Chicoine, Leif D Nelin. 2008. Down Syndrome Patients with Pulmonary Hypertension Have Relatively High Plasma Levels of Asymmetric Dimethylarginine [Abstract]. Pediatric Academic Societies' Annual Meeting [Peer Reviewed] (Published)
• Louis G Chicoine, Mark R Girton, Adam S Feuer, Arturo J Cardounel, Leif D Nelin. 2008. The Role of Monomethyl L-arginine in the Regulation of Pulmonary Vascular Tone in Neonatal Lung [Abstract]. Pediatric Academic Societies' Annual Meeting [Peer Reviewed] (Published)
• Leif D Nelin, Thomas J Calvert, Louis G Chicoine, and Yusen Liu. 2008. Lipopolysaccharide-induced nitric oxide production and hypotension in MKP-1 deficient mice depends on L-arginine uptake [Abstract]. FASEB Journal 2008 (Published)
• Chicoine L, Rodino-Klapac L, Montgomery C, Walton N, Kota J, Coley BD, Clark KR, Mendell JR. 2008. Gastrocnemius Targeted Vascular Delivery of AAV8 in Non-Human Primates [Abstract]. ASGT [Peer Reviewed] (Published)
• Louise R Rodino-Klapac, Chrystal L Montgomery, Nathaniel A. Walton, Janiah Kota, Brian D coley, K. Reed Clark, Jerry R Mendell, Loius G Chicoine. 2008. Gastrocnemius Targeted Vascular Delivery of AAV8 in Non-Human Primates [Abstract]. Pediatric Academic Societies' Annual Meeting [Peer Reviewed] (Published)
• Chicoine LG, Paffett ML, Girton MR, Metropolulus MJ, Joshi MS, Bauer Ja, Nelin LD, Resta TC, Walker BR. 2007. Maturational changes in the regulation of pulmonary vascular tone by nitric oxide in neonatal rats.  Am J Physiol Lung Cell Mol Physiol.. Vol. 293, no. 5. (November): L1261-1270. (IF: 4.137)
• Nelin LD, DT Malleske, MR Stenger, and LG Chicoine. 2007. Vascular arginase and hypertension.  Current Hypertension Reviews. Vol. 3, no. 4. (November): 242-249.
• Pope AJ, Druhan L, Guzman JE, Forbes SP, Murugesan V, Lu D, Xia Y, Chicoine LG, Parinandi NL, Cardounel AJ. 2007. Role of DDAH-1 in lipid peroxidation product-mediated inhibition of endothelial NO generation.  Am J Physiol Cell Physiol.. Vol. 293, no. 5. (November): C1679-1686. (IF: 3.817)
• Rodino-Klapac LR, Janssen PM, Montgomery CL, Coley BD, Chicoine LG, Clark KR, Mendell JR. 2007. A translational approach for limb vascular delivery of the micro-dystrophin gene without high volume or high pressure for treatment of Duchenne muscular dystrophy.  Journal of Translational Medicine. Vol. 5. (September): 45. (IF: 3.508)
• Rodino-Klapac LR, Chicoine LG, Kaspar BK, Mendell JR. 2007. Gene therapy for duchenne muscular dystrophy: expectations and challenges.  Archives of Neurology. Vol. 64, no. 9. (September): 1236-1241. Arch Neurol.. (IF: 7.108)
• Nelin LD, Wang X, Zhao Q, Chicoine LG, Young TL, Hatch DM, English BK, Liu Y. 2007. MKP-1 switches arginine metabolism from nitric oxide synthase to arginase following endotoxin challenge.  Am J Physiol Cell Physiol.. Vol. 293, no. 2. (August): C632-640. (IF: 3.817)
• Chicone Lg, Girton MR, Nelin Ld, and Cardounel AJ. 2007. Developmental differences in pulmonary nitric oxide production and endogenous nitric oxide synthase in the neonatal rat. [Abstract]. FASEB Journal (Published)
• Evans RJ, Young TL, Chicoine LG, and Nelin, LD. 2007. Cytokine-induced L-arginine metabolism depends on cationic amino acid transporter activity. [Abstract]. FASEB Journal (Published)
• Nankervis CA, Preston TJ, Dysart KC, Wilkinson WD, Chicoine LG, Welty SE, Nelin LD. 2007. Assessing heparin dosing in neonates on venoarterial extracorporeal membrane oxygenation.  ASAIO J.. Vol. 53, no. 1. (January): 111-114. 2007 Jan-Feb;53(1):111-4.. (IF: 1.221)
• Cui H, English BK, Chicoine LG, and Nelin LD. 2007. SRC family tyrosine kinases and cytokineinduced arginase expression in pulmonary microvascular endothelial cells. [Abstract]. FASEB Journal (Published)
• Toby I, Chicoine LG, and Nelin LD. 2007. EGFR stimulation promotes profiferation in human pulmonary microvascular endothelial cells. [Abstract]. FASEB Journal (Published)
• Nankervis CA, TJ Preston, KC Dysart, WD Wilkinson, LG Chicoine, SE Welty and LD Nelin. 2007. Activated clotting time versus Factor Xa for determining appropriate heparin dose in neonates on VA ECMO.  ASAIO Journal. Vol. 51: 111-114. (IF: 1.631)
• Stanley KP, Chicoine LG, Yount TL, Reber KM, Lyons CR, Liu Y, Nelin. 2006. Gene transfer with inducible nitric oxide synthase decreases production of urea by arginase in pulmonary arterial endothelial cells.  Am J Physiol Lung Cell Mol Physiol.. Vol. 290, no. 2. (February): L298-306. (IF: 4.137)
• Nelin LD, Chicoine LG, Wang X, Hatch DM, Young TL, and Liu Y. 2006. MKP-1 deficiency results in greater LPS-induced nitric oxide production in Mice [Abstract]. FASEB Journal (Published)
• Chicoine LG, Shilling CJ, Martin P, Kaspar B, McCarty D, Clark KR, Mendell JR. 2006. Regional Gene Delivery with rAAV8 to Lower Limb Sheletal Muscle in Mice [Abstract]. Pediatric Academic Societies' Annual Meeting (Published)
• Chicoine LG, Stenger MR, Metropoulos MJ, Girton MR and Nelin LD. 2006. Maturational changes in NO sensitivity are due to increases in soluable gaunylyl cyclase in the developing rat lung [Abstract]. FASEB Journal (Published)
• Rizo L, Shilling CJ, Haidet A, Umapathi P, Sahenk Z, Chicoine LG, Mendell JR and Kaspar, BK. 2006. rAAV Gene Delivery of Follistatin for Muscle Enhancement in Limb-Girdle Muscular Dystrophy Type 2D [Abstract]. Pediatric Academic Societies' Annual Meeting [Peer Reviewed] (Published)
• Stenger MR, Chicoine LG, Liu Y, Hatch DM, and Nelin LD. 2006. Nitric oxide increases mortality and prevents MKP-1 expression in neonatal mice exposed to hyperoxia [Abstract]. FASEB Journal (Published)
• Nelin LD, Chicoine LG, Reber KM, English BK, Young TL, Liu Y. 2005. Cytokine-induced endothelial arginase expression is dependent on epidermal growth factor receptor.  Am J Respir Cell Mol Biol.. Vol. 33, no. 4. (October): 394-401. (IF: 4.426)
• Nelin LD, Young TL, English BK, Chicoine LG, and Liu Y. 2005. Epidermal growth factor receptor kinase is involved in cytokine-induced arginase, but not nitric oxide synthase, up regulation in bovine pulmonary arterial endothelial cells. [Abstract]. FASEB Jounal (Published)
• Nelin LD, Chicoine LG, English BK, Young TL, and Liu Y. 2005. MKP-1 Switches arginine metabolism from NOS to arginase in RAW264.7 cells. [Abstract]. FASEB Journal (Published)
• Chicoine LG, Young TL, and Nelin LD. 2005. Hypoxia attenuates nitric oxide production in bovine pulmonary arterial endothelial cells transfected with inducible nitric oxide synthase [Abstract]. FASEB Journal (Published)
• Chicoine LG, Young TL, and Nelin LD. 2005. Hypoxia attenuates intric oxide production in bovine pulmonary arterial endothelial cells transfected with inducible nitric oxide synthase [Abstract]. FASEB Journal (Published)
• Chicoine LG, Tzeng E, Bryan R, Saenz S, Paffett ML, Jones J, Lyons CR, Resta TC, Nelin LD, Walker BR. 2004. Intratracheal adenoviral-mediated delivery of iNOS decreases pulmonary vasoconstrictor responses in rats.  J Appl Physiol.. Vol. 97, no. 5. (November): 1814-1822. (IF: 4.232)
• Chicoine LG, Paffett ML, Young TL, and Nelin LD. 2004. Arginase inhibition increases nitric oxide production in bovine pulmonary arterial endothelial cells.  Am J Physiol Lung Cell Mol Physiol.. Vol. 287, no. 1. (July): L60-68. (IF: 4.137)
• Carter BW Jr, Chicoine LG, Nelin LD. 2004. L-lysine decreases nitric oxide production and increases vascular resistance in lungs isolated from lipopolysaccharide-treated neonatal pigs.  Pediatric Res. Vol. 55, no. 6. (June): 979-987. (IF: 2.604)
• Chicoine LG, Paffett ML, Saenz S, Nelin LD. 2004. Differential Regulation of Pulmonary Vasoconstriction by Nitric Oxide in the Developing Rat [Abstract]. Pediatric Research (Published)
• Nelin LD, Carter BW, Stanley KP, Chang R, Chicoine LG. 2004. L-Lysine Decreases Nitric Oxide Production in Lungs Isolated from Lipopolysaccharide Treated Neonatal PIgs. [Abstract]. Pediatric Research (Published)
• Young TL, Chicoine LG, Liu Y, Nelin LD. 2004. Geinistein Inhibits Cytokine-Induced Arginase Expression and Urea Production in Pulmonary Arterial Endothelial Cells [Abstract]. Pediatric Research (Published)
• Chicoine LG, Paffett ML, Saenz S, Bryan R, Resta TC, Nelin LD, Walker BR. 2004. Differential regulation of pulmonary vasoconstriction by nitric oxide in the developing neonate [Abstract]. FASEB Journal (Published)
• LD Nelin, LG Chicoine. 2004. L-arginine, but not d-arginine, increases nitric oxide production and vasodilates hypoxic neonatal pig lungs [Abstract]. FASEB Journal (Published)
• Stanley K, Chicoine LG, and Nelin LD. 2003. Hypoxia Attenuates Nitric Oxide Production in Bovine Pulmonary Arterial Endothelial Cells Transfected with Inducible Nitric Oxide Synthase [Abstract]. Pediatric Research (Published)
• Chicoine LG, Bryan R, Sanez S, Paffett, Nelin LD, and Walker BR. 2003. Tracheal Adenovirus-Mediated iNOS Gene Transfection Attenuates U-46619 Induced Pulmonary Arterial Constriction in Isolated Rat Lungs [Abstract]. Pediatric Research (Published)
• Stanley K, Chang R, Chicoine LG, and Nelin LD. 2003. L-arginine uptake is not affected by iNOS transfection in bovine pulmonary arterial endothelial cells [Abstract]. FASEB Journal (Published)
• Chicoine LG, Bryan R, Sanez S, Paffett, Nelin LD and Walker BR. 2003. Intratracheal Pulmonary Adenoviral iNOS Transfection Decreases Vassoconstrictor Responses in Rats [Abstract]. FASEB Journal (Published)
• Chicoine LG, Avitia JW, Deen C, Nelin LD, Earley S, and Walker BR. 2002. Developmental differences in pulmonary eNOS expression in response to chronic hypoxia in the rat.  J Appl Physiol.. Vol. 93, no. 1. (July): 311-318. (IF: 4.232)
• Earley S, Nelin LD, Chicoine LG, Walker BR. 2002. Hypoxia-induced pulmonary endothelin-1 expression is unaltered by nitric oxide.  J Appl Physiol.. Vol. 92, no. 3. (March): 1152-1158. (IF: 4.232)
• Nelin LD, Krenz GS, Chicoine LG, Dawson CA, and Schapira RM. 2002. L-arginine uptake and metabolism following in vivo silica exposure in rat lungs.  Am J Resp Cell Mol Biol. Vol. 26, no. 3. (March): 348-355. (IF: 4.477)
• Earley S, Nelin LD, Chicoine LG, and Walker BR. 2002. Divergent responses of nitric oxide on hypoxia-induced pulmonary endothelin-1 expression.  J Applied Physiol. (IF: 3.658)
• Stanley KP, Chicoine LG, and Nelin LD. 2002. Inducible nitric oxide synthase and arginase complete for the common substrate L-arginine in bovine pulmonary arterial endothelia cells (bPAEC) [Abstract]. Pediatric Research (Published)
• Carter BW, Chicoine LG and Nelin Ld. 2002. Inhibting arginine uptake decreases Nitric Oxide Production in isolated lungs from Cytokine-t=treated Neonatal pigs [Abstract]. FASEB Journal (Published)
• Nelin LD, Nash HE, Chicoine LG. 2001. Cytokine treatment increases arginine metabolism and uptake in bovine pulmonary arterial endothelial cells.  Am J Physiol Lung Cell Mol Physiol.. Vol. 281, no. 5. (November): L1232-1239. 2001 Nov;281(5):L1232-9.. (IF: 3.924)
• Stanley KP, Chicoine LG, and Nelin LD. 2001. Adenovirus transfection with inducible nitric oxide synthase decreases arginase activity in bovine pulmonary arterial endothelial cells [Abstract]. FASEB Journal (Published)
• Broxterman KJ, Mathew P, Chicoine L. 2000. Left brachial artery thrombus, left axillary vein thrombus, and stroke in a neonate with factor V Leiden mutation.  J Pediatr Hematol Oncol.. Vol. 22, no. 5. (October): 472-475. (IF: 1.176)
• Earley S, Walker BR, and Chicoine LG. 2000. Nitric oxide attenuates hypoxia-inducible factor-mediated endothelin-1 gene expression [Abstract]. FASEB Journal (Published)
• Chicoine LG, Avitia JW, Deen C, Earley S, Nelin LD and Walker BR. 2000. Develomental differences in pulmonary eNOS expression in response to chronic hypoxia in the rat [Abstract]. FASEB Journal (Published)
• Resta TC, Chicoine LG, Omdahl JL, and Walker BR. 1999. Maintained upregulation of pulmonary eNOS gene and protein expression during recovery from chronic hypoxia.  Am J Physiol.. Vol. 276, no. 2 Pt 2. (February): H699-708. 1999 Feb;276(2 Pt 2):H699-708.. (IF: 3.643)
• Resta TC, O?Donaughy TL, Earley S, Chicoine LG, and Walker BR. 1999. Unaltered vasoconstrictor responsiveness after iNOS inhibition in lungs from chronically hypoxic rats.  Am J Physiol.. Vol. 276, no. 1 Pt 1. (January): L122-130. (IF: 3.924)
• Chicoine LG, Lindsey RE, Savich Renate, and Lung D. 1998. Endothelin gene expression does not change with oligohydramnios-induced pulmonary hypoplasia [Abstract]. Pediatric Research (Published)
• Chicoine LG, Resta Tc, Earley S, Lyons RD, Gerard RD, and Walker BR. 1998. Adenovirus-mediated iNOS gene transfection attenuates U-46619 induced pulmonary arterial constriction in isolated rat lungs [Abstract]. Pediatric Research (Published)
• Chicoine Lg, Townsend SF and Stenmark KR. 1995. Insulin-like growth factor binding protein (IGFBP) production changes dramatically in bovine pulmonary artery smooth muscle cells (SMC) during developement [Abstract]. Pediatric Research (Published)
• Chicoine LG, Townsend SF, and Stenmark KR. 1994. Insulin-like growth factor II increases tropoelastin mRNA in neonatal vascular smooth muscle cells (SMCs) via the type 2/mannose-6-phosphate receptor [Abstract]. Clinical Research (Published)
• Chicoine LG, Townsend SF, Badesch DB, and Stenmark KR. 1994. Insulin-like growth factor II )IGF-II) increases tropoelastin mRNA in neonatal vascular smooth muscle cells (SMC's) via two distinct IGF receptors [Abstract]. Pediatric Research (Published)
• Singh P, Reubi JC, Rajakumar G, Guo YS, Prioux H, Chicoine L. 1993. In vivo mitogenic effects of estradiol on colon cancers: role of gastrin and gastrin receptors.  J Steroid Biochem Mol Biol.. Vol. 46, no. 1. (July): 49-60. 1993 Jul;46(1):49-60.. (IF: 2.827)
• Narayan S, Chicione L, Singh P. 1992. Characterization of gastrin binding to colonic mucosal membranes of guinea pigs.  Mol Cell Biochem.. Vol. 112, no. 2. (June): 163-171. (IF: 1.764)
• Singh P, Chicoine LG, Guo Y-S, Narayan S, Rafakumar G, Parekh D, and Townsend CM Jr. 1990. Gastrointestinal Hormone Receptors and Receptor-Regulation. In Gastrointesinal Endocrinology. New York, New York: Academic Press.
• Chicone L, Narayan S, Townsend CM Jr, Singh P. 1989. The presence of a 33-40 KDa gastrin binding protein on human and mouse colon cancer.  Biochem Biophys Res Commun.. Vol. 164, no. 1. (October): 512-519. (IF: 2.648)
• Richman R, Chicoine LG, Collini M, Cook R, and Allis CD. 1988. Micronuclei and the cytoplasm of growing Tetrahymena contain a histone acetylase activity, which is highly specifiec for free histone H4.  Jounal of Cell Biology. Vol. 106, no. 4. (April): 1017-1026. (IF: 9.12)
• Singh P and Chicoine LG. 1988. Species-dependent differences in the molecular size of gastrin receptors by covalent cross-linking studies. [Abstract]. FASEB Journal (Published)
• Chicoine LG, Guo Y-S, Thompson JC, Townsend Jr CM and Singh P. 1988. Regulation of gastin receptor and gastrin levels by gonadal steroids in vivo in rodents [Abstract]. Gastroenterology (Published)
• Chicoine LG, Guo Y-S, Thompson JC, Townsend Jr CM, and Singh P. 1988. Differences in the molecular size of gastrin receptors (GR) on normal and cancerous gut tissues from mice [Abstract]. Gastroenterology (Published)
• Chicoine LG, and Singh P. 1988. Gastrin mediated effects of gonadal steroids on the growth of mouse colon cancer cells in vivo [Abstract]. The 29th National Student Research Forum Publication (Published)
• Chicoine LG, Richman R, Cook R, Gorovsky M, and Allis CD. 1987. A single histone acetyltransferase from Tetrahymena macronuclei catalyzes deposition-related acetylation of free histones and transcription-related acetylation of nucleosomal histones.  Journal of Cell Biology. Vol. 105, no. 1. (July): 127-135. (IF: 9.12)
• Allis D, Chicoine LG, Glover CV, White E, and Gorovsky M. 1986. Enzyme activity dot blots: a rapid and convenient assay for acetyltransferase or protein kinase activity immobilized on nitrocellulose.  Analytical Biochemistry. Vol. 159, no. 1. (November): 58-66. (IF: 3.088)
• Chicoine LG, and Allis CD. 1986. Regulation of histone acetylation during macronuclear differentiation in Tetrahymena: evidence for control at the level of acetylation and deacetylation.  Developmental Biology. Vol. 116, no. 2. (August): 477-485. (IF: 4.416)
• Chicoine LG, Schulman I, Richman R, Cook R, and Allis CD. 1986. Nonrandom utilization of acetylation sites in histones isolated from Tetrahymena. Evidence for functionally distinct H4 acetylation sites. 61(3): 1071-6, 1.  Journal of Biological Chemistry. Vol. 261, no. 3. (January): 1071-1076. (IF: 5.52)
• Allis CD, Chicoine LG, Richman R, and Schulman I. 1985. Deposition-related histone acetylation in micronuclei of conjugating Tetrahymena.  Proceedings of the National Academy of Sciences of the United States of America. Vol. 82, no. 23. (December): 8048-8052. (IF: 10.38)
• Chicoine LG, Wenkert D, Richman R, Wiggins J, and Allis CD. 1985. Modulation of linker histones during development in Tetrahymena: selective elimination of linker histone during the differentiation of new macronuclei.  Developmental Biology. Vol. 109, no. 1. (May): 1-8. (IF: 4.416)
• Allis CD, Allen R, Wiggins J, Chicoine LG and Richman R. 1984. Proteolytic processing of h1-like histones in chromatin: a physiologically and developmentally regulated event in Tetrahymena micronuclei.  Journal of Cell Biology. Vol. 99, no. 5. (November): 1669-1677. (IF: 9.12)
• Louis G. Chicoine, Lecturer. 2011. Treat-Neuromuscular Dystrophy; systemic delivery of AAV for Neuro-muscular gene therapy preclinical round table in the frame for Treat-NMD. Presented at Treat-Muscular Dystrophy, to Genethon - Genocentre. Evry. Paris, France. (December)
• Chicoine, Louis G, Lecturer. 2008. Inhaled Nitric Oxide for the Pre-Term Infant and Medication Safey in the NICU. Presented at Neonatal Nurse Practitioner Regional Education Seminar:, to Non Physician Practitioners- Advanced Practice Nurses. Nationwide Children's Hospital. Columbus, Ohio, USA.
• LG Chicoine. 1999. Adenovirus-mediated iNOS gene transfection attenuates U-46619 induced pulmonary arterial constriction in isolated rat lugns. Presented at Pediatric Academic Societies' Annual Meeting. [Peer Reviewed]
• LG Chicoine, Presenter. 2000. Nitric Oxide attenuates hypoxia-inducible factor-mediated endothelin-1gene expression. Presented at International Experimental Biology Meeting.
• Louis G Chicoine, Presenter. 2003. L-arginine Increases Nitric Oxide Production and Reverses Hypoxic Pulmonary Vasoconstriction in Isolated Perfused Neonatal Pig Lungs. Presented at Pediatric Academic Societies' Annual Meeting. [Peer Reviewed]
• LG Chicoine, Presenter. 1994. Regulators of Fetal Development and Postnatal Function. Insulin-like growth factor II increases tropoelastin mRNA in neonatal vascular smooth muscle cells via two distinct IGF receptors. Presented at Perinatal and Development Medicine Symposium. [Peer Reviewed]
• Louis G Chicoine, Presenter. 2005. Differential Regulation of Pulmonary Vasoconstriction by Nitric Oxide in the Developing Rat. Presented at Pediatric Academic Societies' Annual Meeting. [Peer Reviewed]
• Chicoine LG,. 2009 (January 1). Mice Deficient in MKP-1 develop more severe chronic hypoxia induced pulmonary vascular remodeling. AM J Resp Cell Mol Biol. [Peer Reviewed] (First submission)
• LG Chicoine, Presenter. 2000. Developmental differences in pulmonary eNOS expression in response to chronic hypoxia in the rat. Presented at Pediatric Academic Societies' Annual Meeting and FASEB. [Peer Reviewed]
• Louis G Chicoine, Presenter. 2005. Cytokine-Induced Nitric Oxide Production Is Due Mainly to Inducible Nitric Oxide Synthase in Bovine Pulmonary Arterial Endothelial Cells. Presented at Pediatric Academic Societies' Annual Meeting.
• Chicoine, Louis G, Poster Presenter. 2009. Resveratrol Reverses Both Thromboxane A2 Receptor and Non-Receptor (K+) Mediated Pulmonary Vasocontriction. Presented at Experimental Biology. New Orleans, Louisiana, USA. (April)
• LG Chicoine, Presenter. 1994. Insulin-like growth factor II increases tropoelastin mRNA in neonatal vascular smooth muscle cells via two distinct IGF receptors. Presented at Pediatric Academic Societies' Annual Meeting. [Peer Reviewed]
• Chicoine, Louis G, Lecturer. 2010. L-Arginine Increases Hemodynamically Relevant Nitric Oxide production in Isolated Perfused Lungs From Chronically Hypoxic Rats. Presented at American Pediatric Society - Society of Pediatric Research. Vancouver, British Columbia, Canada. (May)
• LG Chicoine. 1995. Maternal-Fetal-Neonatal-Reproductive for a Research Career in Perinatology. Presented at NICHD Aspen Conference. [Peer Reviewed]
• Chicoine, Louis G, Lecturer. 2011. Exploring the Potential of Plasma Apheresis to Circumvent Pre-Existing Antibodies to AAV8 on Transgene Expression Following Targeted Vascular Delivery. Presented at American Society of Gene and Stem Cell Therapy, to Academic Medical Center physicians and scientists. Seattle, Washington, USA. (May)
• LG Chicoine. 2001. Adenovirus transfection with inducible nitric oxide synthase decreases arginase activity in bovine pulmonary arterial endothelial cells. Presented at International Experimental Biology Meeting. [Peer Reviewed]
• LG Chicone, Presenter. 1995. Insulin-like growth factor binding protein production changes dramatically in bovine pulmonary artery smooth muscle cells during development. Presented at Pediatric Academic Societies' Annual Meeting. [Peer Reviewed]
• Louis G Chicoine, Presenter. 2004. Cytokine-Induced Arginase Expression and Urea Production in Pulmonary Arterial Endothelial Cells. Presented at Pediatric Academic Societies' Annual Meeting. [Peer Reviewed]
• Louis G Chicoine, Presenter. 2005. Differential Regulation of Pulmonary Nitric Oxide Production in the Developing Rat. Presented at International Experimental Biology Meeting. [Peer Reviewed]
• Louis G Chicoine, Presenter. 2003. Tracheal Adenovirus-Mediated iNOS Gene Transfection Attenuates U-46619 Induced Pulmonary Arterial Constriction in Isolated Rat Lungs. Presented at Pediatric Academic Societies' Annual Meeting. [Peer Reviewed]
• Louis G Chicoine, Presenter. 2007. Developmental Differences in Pulmonary Nitric Oxide Production and Endogenous Nitric Oxide Synthase Inhibitors in the Neonatal Rat. Presented at Pediatric Academic Societies' Annual Meeting. [Peer Reviewed]
• Louis G Chicoine, Presenter. 2008. Gastrocnemius Targeted Vascular Delivery of AAV8 in Non-Human Primates. Presented at Pediatric Academic Societies' Annual Meeting. (May) [Peer Reviewed]
• Stenger M, Rose M, Joshi M, Rogers L, Chicoine LG, Welty S, Bauer J, Nelin LD. 2009. Inhaled Nitric Oxide Prevents 3- nitrotyrosine Formation in the Lungs of Neonatal Mice Exposed to Lethal Hyperoxia. AM J Physiol Lung Cell Mol Physiol. [Peer Reviewed] (First submission)
• LG Chicoine, Presenter. 1995. Presenter. Insulin-like growth factor binding protein production changes in bovine pulmonary artery smooth muscle cells during development and in phenotypically distinct adult SMC populations. Presented at 24th Aspen Conference. (September) [Peer Reviewed]
• Louis G Chicoine, Presenter. 2006. Regional Gene Delivery with rAAVB to Lower Limb Skeletal Muscle in Mice. Presented at Pediatric Academic Societies' Annual Meeting. [Peer Reviewed]
• Chicoine, Louis G, Poster Presenter. 2009. Resveratrol Reverses Both Thromboxane A2 Receptor and Non-Receptor (K+) Mediated Pulmonary Vasocontriction. Presented at American Pediatric Society - Society of Pediatric Research. Baltimore, Maryland, USA. (May)
• Louis G Chicoine, Presenter. 2008. MKP-1 Deficiency Augments LPS-Induced NO Production in the Lung. Presented at Pediatric Academic Societies' Annual Meeting. [Peer Reviewed]
• Chicoine, Louis G, Presenter. 2010. Regulation of Endothelial NO Production by PRMT1. Presented at International Symposium on ADMA, to Academic and medical center physicians and scientists. Chicago, Illinois, USA. (November)
• LG Chicoine, Presenter. 1994. Plenary Session. Insulin-like growth factor II increases tropoelastin mRNA in neonatal vascular smooth muscle cells via the type II/mannose-6-phosphate receptor. Presented at Western Society for Pediatric Research (WSPR). [Peer Reviewed]
• Louis G Chicoine, Presenter. 2005. Cytokine-Induced Arginase Expression in Bovine Pulmonary Arterial Endothelial Cells Depends on Src Tyrosine Kinases. Presented at Pediatric Academic Societies' Annual Meeting.
• Louis G Chicone, Presenter. 2005. L-Lysine Decreases Both Nitric Oxide and Urea Production in Bovine Pulmonary Arterial Endothelial Cells Transfected with Inducible Nitric Oxide Synthase. Presented at Pediatric Academic Societies' Annual Meeting. [Peer Reviewed]
• Louis G Chicoine, Presenter. 2006. Maturational Change in NO Sensitivity Are Due to Increases in Soluble Gaunylyl Cyclase in the Developing Rat Lung. Presented at Pediatric Academic Societies' Annual Meeting. [Peer Reviewed]
• Louis G Chicoine, Presenter. 2004. Differential Regulation of Pulmonary Vasoconstriction by Nitric Oxide in the Developing Rat. Presented at Pediatric Academic Societies' Annual Meeting. [Peer Reviewed]