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| Education |
| 2002 |
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Post-Doctoral Fellowship |
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Vascular Biology, Molecular Physiology |
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Davis Heart & Lung Research Institute, The Ohio State University, Columbus, OH |
| 1998 |
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Post-Doctoral Fellowship |
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Molecular and Cell Biology |
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The Ohio State University, Dept. of Internal Medicine, Columbus, OH |
| 1997 |
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PhD |
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Molecular, Cellular & Developmental Biology |
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The Ohio State University, Columbus, Ohio |
| 1988 |
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BA |
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Biology |
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The College of Wooster, Wooster, OH |
| Professional Experience |
| 2008- PRES |
Principal Investigator, Center for Cardiovascular and Pulmonary Research, The Research Institute at Nationwide Children’s Hospital, Columbus, Ohio |
| 2002- 2008 |
Research Scientist, Davis Heart & Lung Research Institute, The Ohio State University |
| 1998- 2002 |
Postdoctoral Researcher/Fellow, Davis Heart & Lung Research Institute, The Ohio State University |
| 1997- 1998 |
Postdoctoral Researcher, Department of Internal Medicine, The Ohio State University |
| 1990- 1997 |
Graduate Research Associate, Program in Molecular, Cellular & Developmental Biology, The Ohio State University, Columbus, Ohio |
| 1988- 1990 |
Jr. Research Associate, Department of Psychiatry, MetroHealth Medical Center, Cleveland, Ohio |
| 1988- 1988 |
Laboratory Technician, Department of Preventive Veterinary Medicine, Food Animal Health Research Program, Ohio Agriculture Research & Development Center, Wooster, Ohio |
Research Interests
My research interests and activities are currently supported by Institutional internal funds, the American Heart Association, and the National Heart, Lung, and Blood Institute of the National Institutes of Health. The funds support research examining mechanisms of expression and cell-surface mobilization of endogenous micro-vascular smooth muscle G protein-coupled α2C-adrenergic receptors (α2C-ARs).
My colleagues and I identified the α2C-ARs as “stress-receptors” of the vascular sympathetic system. We have recently identified a novel signaling cascade in human vascular smooth muscle cells involving cyclic AMP, the Ras-related small G protein Rap1, actin binding protein filamin2, α2C-ARs, and the actin cytoskeleton. Current and future work involves studies to understand the role of Rap1 in the cardiovasculature and examination of stress-induced signature microRNAs and proteins in vascular smooth muscle cells. These studies will potentially lead to identification of physiologically relevant "vasculo-protective" players, conferring "stress-tolerance" to cells during cellular stress or vascular injury.
Broadly, these studies involve understanding the mechanisms behind alterations in peripheral vascular resistance, and peripheral vascular disorders. For example, in the cerebral circulation, in stroke, diabetes, hypercholesterolemia, hypertension, hypothermic response of the microvasculature seen in Raynaud’s phenomenon, secondary Raynaud’s associated with the devastating disease scleroderma, and hand-arm vibration syndrome.
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| Selected Publications |
| Eid AH, Chotani MA, Mitra S, Miller TJ, Flavahan S, Flavahan NA. 2008. Cyclic AMP acts through Rap1 and JNK signaling to increase expression of cutaneous smooth muscle α2C-adrenoceptors. Am J Physiol. Heart Circ Physiol. 295: H266-H272. PubMed ID: 18487435 |
| Eid A H, Maiti K, Mitra S, Chotani MA, Flavahan S, Bailey SR, Thompson-Torgerson CS, Flavahan NA. 2007. Estrogen increases smooth muscle expression of α2C-adrenoceptors and cold-induced constriction of cutaneous arteries. Am J Physiol. Heart Circ Physiol. 293: H1955-H1961. PubMed ID: 17644575 |
| Bayoumi MM, Alkheraije KA, El-Sayed O, Wisel S, Sarker K, Chotani MA, Zweier JL, Nuovo G, Goldschmidt-Clermont PJ, Hassanain H. 2007. Vascular hypertrophy caused by transgenic overexpression of profilin 1. J Biol Chem. 282:37632-37639. PubMed ID: 17942408 |
| Martin MM, Buckenberger JA, Jiang J, Malana GE, Nuovo GJ, Chotani M, Feldman DS, Schmittgen TD, Elton TS. 2007. The human angiotensin II type 1 receptor +1166 A/C polymorphism attenuates microRNA-155 binding. J Biol Chem. 282: 24262-24269. PubMed ID: 17588946 |
| Chotani MA, Mitra S, Eid AH, Han SA, Flavahan NA. 2005. Distinct cyclic AMP signaling pathways differentially regulate α2C-adrenoceptor expression: role in serum induction in human arteriolar smooth muscle cells. Am. J. Physiol. Heart Circ. Physiol, special “Translational Physiology” series article; 288: H69-H76. PubMed ID: 15345481 |
| Chotani MA, Mitra S, Su B Y, Flavahan S, Eid AH, Clark KR, Montague C, Paris H, Handy D E, Flavahan NA. 2004. Regulation of α2C-adrenoceptors in human vascular smooth muscle cells. Am. J. Physiol. Heart Circ. Physiol, special “Translational Physiology” series article; 286: H59-H67. PubMed ID: 12946937 |
| Chotani MA, Flavahan S, Mitra S, Daunt D, Flavahan NA. 2000. Silent α2C-adrenergic receptors enable cold-induced vasoconstriction in cutaneous arteries. Am. J. Physiol. Heart Circ. Physiol. 278:H1075-H1083. PubMed ID: 10749700 |
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