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HARPER LABORATORY
Dr. Harper's laboratory studies gene therapy for dominant genetic diseases using RNA interference (RNAi), with particular focus on muscular dystrophy and neurodegenerative disease. The Harper lab utilizes a broad range of tools, including molecular biology, biochemistry, RNAi, gene therapy vectors, and animal models for muscular dystrophy and peripheral neuropathy.
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| Education |
| 2007 |
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Post-doctoral |
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Gene Therapy/RNAi |
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University of Iowa College of Medicine, Iowa City, IA |
| 2002 |
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Ph.D. |
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Cellular and Molecular Biology |
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University of Michigan Medical School, Ann Arbor, MI |
| 1996 |
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BS |
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Biology |
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Saginaw Valley State University, Saginaw, MI |
HARPER LABORATORY STAFF
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Principal Investigator:
Scott Harper, Ph.D.
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| Selected Publications |
| S.Q. Harper. Progress and challenges in RNAi therapy for Huntington's Disease. (2009) Archives of Neurology |
| S.Q. Harper and Gonzalez-Alegre P. Lentivirus-mediated RNA interference in mammalian neurons. (2008) Methods in Molecular Biology 442, 95-112. PubMed ID: 18369781 |
| A. Packer, Y. Xing, S.Q. Harper, L. Jones, and B.L. Davidson. The bi-functional microRNA mir9/mir9* regulates REST and coREST and is down-regulated in Huntington's desease (2008) Journal of Neuroscience, 28(53):14341-6 PubMed ID: 19118166 |
J.L. McBride*, R.L. Boudreau*, S.Q. Harper* (shared first authorship), A. Mas Monteys, P.D. Staber, I. Martins, B. Gilmore, H. Burstein, R.W. Peluso, B. Polisky, B.J. Carter, and B.L. Davidson. MicroRNA shuttles mitigate short-hairpin RNA mediated toxicity in the brain: Implications for therapeutic development of RNA interference. (2008) Proceedings of the National Academy of Sciences, USA 105(15): 5868-73. PubMed ID: 18398004 |
P. Allaire, B. Ritter, S. Thomas, J. Burman, A. Denisov, V. Legendre-Guillmen, S.Q. Harper, B.L. Davidson, K. Gehring, and P. McPherson. Connecdenn, A DENN domain-containing protein functioning in synaptic vescle endocytosis. (2006) Journal of Neuroscience 26(51) 13202-13212 PubMed ID: 17182770 |
| S.Q. Harper, P.D. Staber, C.R. Beck, S.K. Fineberg, C.S. Stein, D. Ochoa, and B.L. Davidson. Optimization of feline immunodeficiency viral vectors for RNA interference. (2006), Journal of Virology 80(19), 9371-9380 PubMed ID: 16973543 |
| S.Q. Harper, P.D. Staber, X. He, S.L. Eliason, I.H. Martins, L. Yang, H.L. Paulson, R.M. Kotin, and B.L. Davidson. RNA interference improves behavioral and neuropathological abnormalities in a mouse model for Huntington’s disease. (2005) Proceedings of the National Academy of Sciences, USA. 102(16), 5820-5825. PubMed ID: 15811941 |
| S.Q. Harper and B.L. Davidson. Plasmid-based RNA interference. (2005) Methods in Molecular Biology 309, 219-235. PubMed ID: 15990403 |
| B.L. Davidson and S.Q. Harper. Viral delivery of short hairpin RNAs. (2005) Methods in Enzymology 392, 145-173. PubMed ID: 15644180 |
| M. Liu, Y. Yue, S.Q. Harper, R.W. Grange, J.S. Chamberlain, and D. Duan. Adeno-associated virus-mediated microdystrophin expression protects young mdx muscle from contraction-induced injury. (2005) Molecular Therapy 11(2), 245-256. PubMed ID: 15668136 |
| H. Xia, Q. Mao, S.L. Eliason, S.Q. Harper, I.H. Martins, H.T. Orr, H.L. Paulson, L. Yang, R.M. Kotin, and B.L. Davidson. RNAi suppresses polyglutamine-induced neurodegeneration in a model of spinocerebellar ataxia. (2004) Nature Medicine 10(8), 816-820. PubMed ID: 15235598 |
| M.J. Blankinship, P. Gregorovic, J.M. Allen, S.Q. Harper, H.A. Harper, C. Halbert, A.D. Miller, and J.S. Chamberlain. Efficient transduction of skeletal muscle using vectors based on adeno-associated virus serotype-6. (2004) Molecular Therapy 10(4), 671-678. PubMed ID: 15451451 |
| Y. Yue, Z. Li, S.Q. Harper, R.L. Davisson, J.S. Chamberlain, and D. Duan. Microdystrophin gene therapy of cardiomyopathy restores dystrophin-glycoprotein complex and improves sarcolemma integrity in the mdx mouse heart. (2003) Circulation 108(13), 1626-1632. PubMed ID: 12952841 |
| S.Q. Harper, M. Hauser, C. DelloRusso, D. Duan, R.W. Crawford, S. Phelps, H.A. Harper, A.S. Robinson, J.F. Engelhardt, S.V. Brooks, and J.S. Chamberlain. (2002) Modular flexibility of dystrophin: Implications for gene therapy of DMD. Nature Medicine, 8(3), 253-261. PubMed ID: 11875496 |
| S.Q. Harper, R.W. Crawford, C. DelloRusso, and J.S. Chamberlain. Spectrin-like repeats from dystrophin and ?-actinin are not functionally interchangeable. (2002) Human Molecular Genetics, 11(16), 1807-1815. PubMed ID: 12140183 |
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