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YU LABORATORY
Currently we are investigating the details of gene copy number variations in human autoimmune, neurological and infectious diseases. We are funded by the NIH to study the genetic and clinical risk factors of human systemic lupus erythematosus (SLE). We are a member of the Ohio SLE Study Group that consists of four research teams headed by Dr. Lee Hebert, Dr. Brad Rovin, Dr. Dan Birmingham and Dr. Chack-Yung Yu.
Our laboratory is specialized in molecular genetic analyses of human complex diseases. Our lab is recognized for the characterization of the RCCX modules and gene copy number variations of human complement components C4A and C4B. We apply a variety of genotyping and phenotyping techniques in our research projects, which include real-time PCR, genomic restriction fragment length polymorphism, DNA sequencing, pulsed field gel electrophoresis, immunofixation, immunodiffusion and flow cytometry.
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| Education |
| 1989 |
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Post Doctoral Fellowship |
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Molecular Immunology |
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MRC Laboratory of Molecular Biology, Cambridge, UK |
| 1986 |
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D.Phil. |
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Biochemistry |
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Trinity College, Oxford University, Oxford, UK |
| 1983 |
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M.Phil. |
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Molecular Biology |
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The Chinese University of Hong Kong, Hong Kong |
| 1981 |
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B.Sc. |
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Biology and Biochemistry |
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The Chinese University of Hong Kong, Hong Kong |
| Professional Experience |
| 2006- PRES |
Proessor, Department of Pediatrics, Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University |
| 1991- PRES |
Faculty Member, Molecular, Cellular and Development Biology Program, The Ohio State University |
| 1990- PRES |
Principal Investigator, The Research Institute at Nationwide Children's Hospital |
| 1996- 2006 |
Associate Professor, Department of Pediatrics, Department of Molecular Virology, Immunology and Medical Genetics, The Ohio State University |
| 1990- 1996 |
Assistant Professor, Pediatrics, Medical Microbiology and Immunology, The Ohio State University |
| 1987- 1990 |
Research Fellow and Non-Clinical Scientific Staff, MRC Laboratory of Molecular Biology, Cambridge,U.K. (with Dr. Cesar Milstein, Nobel laureate, C.H., F.R.S.) |
| 1983- 1987 |
Research Student, Medical Research Council (MRC) Immunochemistry Unit, Oxford University (with the late Prof. Rodney R. Porter, Nobel laureate, C.H., F.R.S. and Dr. R. Duncan Campbell) |
Research Interests
My primary research interests are focused on the structural, functional and genetic variations of immune effector proteins such as complement proteins, immunoglobulin receptors, and gene products encoded by the major histocompatibility complex in the immune defense and autoimmunity.
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YU LABORATORY STAFF
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Principal Investigator:
Chack-Yung Yu, D.Phil.
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| Selected Publications |
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Saxena K, Kitzmiller KJ, Wu YL, Zhou B, Esack N, Hiremath L, Chung EK, Yang Y, Yu CY. Great genotypic and phenotypic diversities associated with copy-number variations of complement C4 and RP-C4-CYP21-TCX (RCCX) modules: a comparison of Asian-Indian and European American populations. Mol Immunol (2009, Jan 8)
PubMed ID: 19135723 |
Wu YL, Yang Y, Chung EK, Zhou B, Kitzmiller KJ, Savelli SL, Nagaraja HN, Birmingham DJ, Tsao BP, Rovin BH, Hebert LA and Yu CY. Phenotypes, genotypes and disease susceptibility associated with gene copy number variations: complement C4 CNVs in European American healthy subjects and those with systemic lupus erythematosus. Cytogenet Genome Res 123 (2009, in press). |
Wu YL, Hauptmann G, Viguier M, YU CY. (2009) Molecular basis of complete complement C4 deficiency in two North-African families with systemic lupus erythematosus. Genes Immun. 10(5): 433-45 Epub 2009 Mar 12.
PubMed ID: 19279649 |
Cohen RA, Bayliss G, Crispin JC, Kane-Wanger GF, Van Beek CA, Kyttaris VC, Avalos I, Yu CY, Tsokos GC and Stillman IE. T cells and in situ cryoglobulin deposition in the pathogenesis of lupus nephritis. Clin Immunol 128:1-7, 2008. PubMed ID: 18565470 |
Wu YL , Savelli SL, Yang Y, Zhou B, Rovin B, Birmingham DJ, Nagaraja HN, Hebert LA and Yu CY. Sensitive and specific real-time PCR assays to accurately determine copy number variations (CNVs) of human complement C4A, C4B, C4-long, C4-short and RCCX modules: Elucidation of C4 CNVs in 50 consanguineous subjects with defined HLA genotypes. J Immunol 179: 3012-3025, 2007. PubMed ID: 17709516 |
Auer H, Newsom DL, Nowak NJ, McHugh KM, Singh S, Yu CY, Yang Y, Wenger GD, Gastier-Foster JM and Kornacker K: Gene-resolution analysis of DNA copy number variation using oligonucleotide expression microarrays. BMC Genomics 8:111, 2007. PubMed ID: 17470268 |
Yang Y, Chung EK, Wu YL, Savelli S, Nagaraja HN, Zhou B, Hebert M, Jones KN, Shu Y, Kitzmiller K, Blanchong CA, McBride K, Higgins GC, Rennebohm RM, Rice RR, Hackshaw KV, Roubey RA, Grossman JM, Tsao BP, Birmingham DJ, Rovin BH, Hebert LA and Yu CY: Gene copy number variation and associated polymorphisms of complement component C4 in human systemic lupus erythematosus (SLE): Low copy number is a risk factor for, high copy number is a protective factor against, European American SLE disease susceptibility. Am J Hum Genet 80:1037-1054, 2007. PubMed ID: 17503323 |
Wu H, Boackle SA, Hanvivadhanaku P, Ulgiati D, Grossman JM, Lee Y, Shen N, Abraham LJ, Mercer TR, Park E, Hebert LA, Rovin BH, Birmingham DJ, Chang D, Chen CJ, McCurdy D, Badsha HM, Thong BYH, Chng HH, Arnett FC, Wallace DJ, Yu CY, Hahn BH, Cantor RM, Tsao BP: Association of a common complement receptor 2 haplotype with increased risk of SLE. Proc Natl Acad Sci USA 104:3961-3966, 2007. PubMed ID: 17360460 |
| Yu CY, Hauptmann G, Yang Y, Wu YL, Birmingham DJ, Rovin BH and Hebert LA: Complement deficiencies in human systemic lupus erythematosus (SLE) and SLE nephritis: epidemiology and pathogenesis. In: Tsokos GC, Gordon C and Smolen JS (eds) Systemic Lupus Erythematosus: a Companion to Rheumatology. Philadelphia: Elsevier, pp183-193, 2007. |
Chung EK, Wu YL, Yang Y, Zhou B and Yu CY. Human complement components C4A and C4B: complex genotypes and phenotypes. Cur Protoc Immunol, Unit 13.8.1-13.8.36, 2005. PubMed ID: 18432942 |
Rovin BH, Song H, Birmingham DJ, Hebert LA, Yu CY, and Nagaraja HN: Urine chemokines as biomarkers of human SLE activity. J Am Soc Nephrol 16: 467-473, 2005. PubMed ID: 15601744 |
Yu CY, Whitacre CC: Sex, MHC and complement C4 in autoimmune diseases. Trends Immunol 25:694-699, 2004. PubMed ID: 15530841 |
Yang Y, Lhotta K, Chung EK, Eder P, Neumair F and Yu CY: Complete complement components C4A and C4B deficiencies in human kidney diseases and systemic lupus erythematosus. J Immunol 173:2803-2814, 2004. PubMed ID: 15294999 |
Yang Y, Chung EK, Zhou B, Lhotta K, Hebert LA, Birmingham DJ, Rovin BH and Yu CY: The intricate role of complement C4 in human SLE. Curr Direct Autoimmun 7:98-132, 2004.
PubMed ID: 14719377 |
Yang Y, Chung EK, Zhou B, Blanchong CA, Yu CY, Füst G, Kovács M, Vatay A, Szalai C, Karádi I and Varga L: Diversity in intrinsic strengths of the human complement system: serum C4 protein concentrations correlate with C4 gene size and polygenic variations, hemolytic activities and body mass index. J Immunol 171:2734-2745, 2003. PubMed ID: 12928427 |
Yu CY, Chung EK, Yang Y, Blanchong CA, Jacobsen N, Saxena K, Yang Z, Miller W, Varga L and Fust G: Dancing with complement C4 and the RP-C4-CYP21-TNX (RCCX) modules of the major histocompatibility complex. Progr Nucl Acid Res Mol Biol 75:217-292, 2003. PubMed ID: 14604014 |
Chung EK, Yang Y, Rennebohm RM, Lokki ML, Higgins GC, Jones KN, Zhou B, Blanchong CA and Yu CY: Genetic sophistication of human complement C4A and C4B and RP-C4-CYP21-TNX (RCCX) modules in the major histocompatibility complex (MHC). Am J Hum Genet 71:823-837, 2002. PubMed ID: 12226794 |
Blanchong CA, Zhou B, Rupert KL, Chung EK, Jones KN, Sotos JF, Rennebohm RM and Yu CY: Deficiencies of human complement component C4A and C4B and heterozygosity in length variants of RP-C4-CYP21-TNX (RCCX) modules in Caucasians: the load of RCCX genetic diversity on MHC-associated disease. J Exp Med 191:2183-2196, 2000.
PubMed ID: 10859342 |
Yu CY, Yang Z, Blanchong CA and Miller W: The human and mouse MHC class III region: a parade of the centromeric segment with 21 genes. Immunol Today 21:320-328, 2000. PubMed ID: 10871871 |
Yang Z, Mendoza AR, Welch TR, Zipf WB and Yu CY: Modular variations of HLA class III genes for serine/ threonine kinase RP, complement C4, steroid 21-hydroxylase CYP21 and tenascin TNX (RCCX): a mechanism for gene deletions and disease associations. J Biol Chem 274:12147-12156, 1999. PubMed ID: 10207042 |
Yang Z, Shen L, Dangel AW, Wu LC and Yu CY: Four ubiquitously expressed genes, RD-SKI2W-DOM3Z-RP1, are present between complement component genes factor B and C4 in the class III region of the HLA. Genomics 53: 338-347, 1998. PubMed ID: 9799600 |
Qu XD, Yang Z, Zhang S, Shen L, Dangel A., Redman KL, Hughes JH, Wu LC and Yu CY: The human HLA DEVH-box protein Ski2w is associated with polysomes and ribosomes. Nucleic Acids Res 26: 4068-4077, 1998. PubMed ID: 9705521 |
Dangel AW, Shen L, Mendoza AR, Wu, LC and Yu CY (1995) Human helicase gene SKI2W in the HLA class III region exhibits striking similarities to the yeast antiviral gene SKI2 and to the human gene KIAA0052: emergence of a new gene family. Nucleic Acids Res. 23:2120-2126. PubMed ID: 7610041 |
Dangel AW, Mendoza AR, Baker BJ, Daniel CM, Carroll MC, Wu LC and Yu CY: The dichotomous size variation of human complement C4 gene is mediated by a novel family of endogenous retroviruses which also establishes species-specific genomic patterns among Old World primates. Immunogenetics 40:425-436,1994. PubMed ID: 7545960 |
Shen L, Wu LC, Sanlioglu S, Chen R, Mendoza AR, Dangel AW, Carroll MC, Zipf WB and Yu CY: Structure and genetics of the partially duplicated gene RP located immediately upstream of the complement C4A and the C4B genes in the HLA class III region: molecular cloning, exon-intron structure, composite retroposon, and breakpoint of gene duplication. J Biol Chem 269:8466-8476, 1994. PubMed ID: 8132574 |
Yu CY: The complete exon-intron structure of a human complement component C4A gene: DNA sequences, polymorphism, and linkage to the 21-hydroxylase gene. J Immunol 146:1057-1066, 1991. PubMed ID: 1988494 |
Yu CY and Milstein C: A physical map linking the five CD1 human thymocyte differentiation antigen genes. EMBO J. 8:3727-3732, 1989. PubMed ID: 2583117 |
Yu CY, Campbell RD and Porter RR: A structural model for the location of the Rodgers and the Chido antigenic determinants and their correlation with the human complement C4A/C4B isotypes. Immunogenetics 27:399-405, 1988. PMID: 2453459 PubMed ID: 2453459 |
Yu CY, Belt KT, Giles CM, Campbell RD and Porter RR: Structural basis of the polymorphism of human complement component C4A and C4B: gene size, reactivity and antigenicity. EMBO J 5:2873-2881, 1986. PubMed ID: 2431902 |
| Belt KT, Yu CY, Carroll MC and Porter RR: Polymorphism of human complement component C4. Immunogenetics 21:173-180, 1985. PubMed ID: 3838531 |
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