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BRIGSTOCK LABORATORY
We are studying the role of connective tissue growth factor (CTGF) in fibrotic diseases, which are characterized by excessive production and deposition of insoluble collagen matrix, resulting in loss of tissue structure and organ dysfunction. Our main research efforts are currently focussed on liver and pancreatic fibrosis but we have collaborative efforts that focus on other organ systems such as the lung, intestine, heart and skin. Using molecular, cellular and biochemical approches, both in vitro and in vivo, our goals are to understand the mechanisms by which CTGF stimulates collagen production by fibrogenic cells types. In this manner, we will determine the therapeutic value of targetting pathways of CTGF biosynthesis or action to treat a variety of fibrotic disorders.
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| Education |
| 1991 |
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Post-Doc |
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Cell Biology |
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Harvard Medical School, Boston MA |
| 1988 |
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Ph.D. |
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Biochemistry |
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The Babraham Institute & Cambridge University, UK |
| Professional Experience |
| 2008- PRES |
Professor of Surgery
Division of Pediatric Surgery
The Ohio State University College of Medicine |
| 1993- PRES |
Member, Molecular, Cellular and Developmental Biology Program,The Ohio State University |
| 1991- PRES |
Principal Investigator
The Research Institute at Nationwide Children’s Hospital |
| 1991- PRES |
Director, Pediatric Surgery Research Laboratory,
Ohio State University College of Medicine |
| 1997- 2008 |
Associate Professor of Surgery
Division of Pediatric Surgery
The Ohio State University College of Medicine |
| 1991- 1997 |
Assistant Professor of Surgery
Division of Pediatric Surgery
The Ohio State University College of Medicine |
| 1988- 1991 |
Post-doctoral Research Fellow
Department of Surgery
Harvard Medical School and Children's Hospital, Boston MA |
| 1984- 1988 |
Graduate Student
Institute of Animal Physiology and Cambridge University, UK |
BRIGSTOCK LABORATORY STAFF
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Principal Investigator:
David R. Brigstock, Ph.D.
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| Selected Publications |
| Crawford LA, Guney MA, Oh YA, Deyoung RA, Valenzuela DM, Murphy AJ, Yancopoulos GD, Lyons KM, Brigstock DR, Economides A, Gannon M (2009) Connective tissue growth factor (CTGF) inactivation leads to defects in islet cell lineage allocation and β cell proliferation during embryogenesis. Mol Endocrinol 23:324-36. |
| Lawrenica C, Charrier A, Huang G and Brigstock DR (2009) Ethanol-mediated expression of connective tissue growth factor (CCN2) in mouse pancreatic stellate cells Growth Factors 27 91-99 |
| Wang J, Leclercq, Brymore JM, Ramezani-Mogadam M, London RM, Brigstock DR, George J (2009). Kupffer cells mediate leptin-induced liver fibrosis Gastroenterology 137 713-723 |
| Brigstock DR (2009) Strategies for blocking the fibrogenic actions of connective tissue growth factor (CCN2): From pharmacological inhibition in vitro to targeted siRNA therapy in vivo. J. Cell Comm Signal 3 5-18 |
| Tong Z, Chen R, Kemper S, Perbal B, and Brigstock DR (2009) Susceptibility to liver fibrosis in mice expressing a connective tissue growth factor transgene in hepatocytes Hepatology 50 939-947 |
| Gao R and Brigstock DR (2009) Connective tissue growth factor hammerhead ribozyme attenuates human hepatic stellate cell function. World J Gastroenterology 15 3807-3813 |
| Brigstock DR (2009) Connective tissue growth factor (CCN2, CTGF) and organ fibrosis: Lessons from transgenic animals. J. Cell Comm. Signal http://dx.doi.org/1007/s12079-009-0071-5 |
| Leask A, Chen S, Pala D, and Brigstock DR (2008) Regulation of CCN2 expression and promoter activity in activated hepatic stellate cells. J. Cell Comm. 2 49-56 |
| Tong Z, Brigstock, D.R. (2006) Intrinsic biological activity of the thrombospondin structural homology repeat in connective tissue growth factor J. Endocrinol 188 R1-R8 |
| Gao, R. and Brigstock, D.R. (2006) A novel integrin ?5?1–binding domain in module 4 of connective tissue growth factor promotes adhesion and migration of activated pancreatic stellate cells. Gut 55 856-862. |
| Gao R and Brigstock DR (2005) Connective tissue growth factor (CCN2) in rat pancreatic stellate cell function: Integrin ?5?1 as a novel CCN2 receptor. Gastroenterology 129 2019-2030 |
| Gao R, Ball DK, Perbal B, Brigstock DR (2004) Connective tissue growth factor (CCN2) induces c-fos gene activation and cell proliferation through p44/42 MAP kinase (ERK1/2) in primary rat hepatic stellate cells J. Hepatol 40 431-438 |
| Gao R., Brigstock DR (2004) Connective tissue growth factor (CCN2) induces adhesion of rat activated hepatic stellate cells by binding of its carboxyl-terminal domain to integrin ?v?3 and heparan sulfate proteoglycans. J Biol Chem 279 8848-8855 |
| Ball, D.K., Moussad, E.E-D.A., Rageh, M.A.E., Kemper, S.A, Brigstock, D.R. (2003) Establishment of a recombinant expression system for connective tissue growth factor (CTGF) that models CTGF processing in utero. Reproduction 125 271-284 |
| Wang JF, Olson ME, Ball DK, Brigstock DR, Wright JB, Hart DA (2003). Recombinant connective tissue growth factor modulates porcine skin fibroblast gene expression.Wound Repair Regen 11 220-229 |
| Gao R, Brigstock DR (2003) Low-density lipoprotein receptor-related protein is a heparin-dependent adhesion receptor for connective tissue growth factor (CTGF) in rat activated hepatic stellate cells. Hepatol Res 27 214-220 |
| Ball DK, Wilson AW, Kemper SA, Brigstock DR (2003). The heparin-binding 10kDa fragment of connective tissue growth factor (CTGF) containing module 4 alone stimulates cell adhesion. J Endocrinol 176 R1-R7 |
| di Mola FF, Friess H, Riesle E, Koliopanos A, Buchler P., Zhu Z, Brigstock DR, Korc M, Buchler MW (2002) Connective tissue growth factor (CTGF) is involved in pancreatic repair and tissue remodeling in human and rat acute necrotizing pancreatitis. Annals Surgery 235 60-67 |
| Williams, E.J., Gaca, M.D.A., Brigstock, D.R., Arthur, M.J.P, and Benyon, R.C. (2000) Increased expression of connective tissue growth factor in fibrotic human liver and in activated hepatic stellate cells. J. Hepatology 32 754-761 |
| Brigstock, D.R., Steffen, C.L. Kim, G.Y., Vegunta, R.K. Diehl, J.R. and Harding P.A. (1997) Purification and characterization of novel heparin-binding growth factors in uterine secretory fluids: Identification as heparin-regulated 10,000-Mr forms of connective tissue growth factor. J. Biol. Chem.272 20275-20282 |
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