Flow Cytometry Core Service Request
The BD FACS Calibur cytometer has two lasers, 488 and 633, 2 scatter detectors, and 4 PMTs capable of 4 color analysis with the two excitation wavelengths mentioned. 3 color manual compensation is available. The Calibur uses BD Cell Quest Pro acquisition and analysis software. For additional information on the use of the Calibur go to the Calibur User Guidelines for step by step instructions or Calibur Quick Notes for a condensed operational guide.
The flow core has three BD LSR II cytometers available for your use. Starting with the Dodecapus and progressing to either the Behemoth or the Octopus each of these cytometers offers increased capacity. Additionally, since all three cytometers utilize DIVA acquisition software there is a level of redundancy across all three machines assuring that regardless of schedule conflicts or machine downtime one should have access to one machine or another at any time. All LSR’s have automatic compensation capability. For additional information on the use of the LSR’s go to the Filter Configurations and/or LSR User Guidelines for in depth, step by step instructions. You can also access the LSR Quick Notes for a condensed version on how to operate the LSR II.