Flow Cytometry Core Service Request
Faculty, staff, and students of The Research Institute at Nationwide Children’s research community are permitted to use the Flow Cytometry Facility. Prior to independent use of any of the cytometers every user is required to attend an in-service training session with one of the core facility staff.
Flow Cytometry sessions on any of the analytical cytometers are scheduled on a first come first serve basis by signing up via the Flow Cytometry core service request link. Access to the eCores reservation system is predicated upon completion of the required initial training session for all new cytometer users. In addition you must have an eCores account in order to access the reservation system. All cytometers are available to investigators 24 hours/day, 7 days/week. Once the required training session has been completed new users will be provided with a user name and password in order to use the cytometers. Please be on time for appointments and do not schedule more time than what is reasonably necessary to run samples. Run times vary depending on the experiment; however, 60-90 seconds per tube is generally a good estimate for the amount of time to schedule. In the event that you won’t use your scheduled time please make every effort to cancel your reservation such that other users may use the machine. Users that habitually fail to notify the facility of cancellations will be given a written warning. If the problem persists, the investigator will be charged for unused appointments.
Cell sorting is performed by core personnel. To make an appointment for cell sorting please contact us via phone or e-mail to set up a time. Please respect the time of the core personnel and other investigators by having your Properly Prepared Samples ready to go at the scheduled time. As with the cytometers, users that habitually fail to inform the staff of cancellations will be charged for unused appointments. Note that all sorts will incur one additional hour of charges for set-up time.
It is also required that each principal investigator complete a Sort Questionnaire for each and every sort protocol.
The Flow Cytometry Core does not operate for profit. Users are charged for actual usage time in five minute increments with a minimum 15 minute charge. Charges vary depending upon the machine used and whether or not the principal investigator is on staff at The Research Institute at Nationwide Children’s. All cytometers have a log-in procedure from which usage is calculated so be aware that once logged in charges are accruing. Turning the machines on and running Quality Control acquisitions does not require individual investigator log-in so charges are not accrued for these periods of time. Listed below is the current menu of charges for each application.
|Cytometer Usage||In House||Out of House|
|Cell Sorting*||In House||Out of House|
* In addition to the hourly charge for actual sorting, there is an additional hour added to all sorts for setup time.
The Flow Cytometry Core supports the use of Flow Jo analysis software for analyzing your data. Web based tutorials in addition to printed materials are available in order to learn the use of this data analysis software. The core has provided various centers throughout the institution with the required Flow Jo dongles in order to be able to use the software. These dongles are to be shared by investigators within their center. In addition the core has several loaner dongles that can be checked out for short periods of time should the need arise. Finally, the core also has two MAC/Flow Jo enabled computers for public use. These are located near the core in the cubicle adjacent to W285. Contact RIS to have the latest version of the Flow Jo software loaded onto your computer be it PC or Mac.
Investigators are ultimately responsible for their own data management. Investigators may store their data on the individual cytometer computers; however this is not a long term solution. Users should transfer their data files either to the recommended media of a flash drive or to disc. While storage on the cytometer computers is allowed, it is recommended that investigators make it a routine practice to copy their data at the end of each session so as to avoid any data loss due to a crashed computer etc.
Although it is the responsibility of each investigator to handle their own data management, flow cytometry data generated at the flow core facility is archived in duplicate by facility personnel on a regular basis. Archived data can be retrieved upon request when investigators provide dates and descriptions of individual samples, and identify which flow cytometer was used for acquisition. However, this should be a rarely used service if you are managing your own data as noted above. Data from each cytometer is generally archived on a quarterly or biannual basis depending primarily upon individual computer hard drive space and performance. Once data has been archived to disc, all copied files are deleted from the hard drive of the computers. For this reason it is also suggested that investigators create folders of templates for often used experimental set-ups. Template folders and their individual non-data containing templates will not be erased. Prior to the back-up and deletion of data, investigators will be sent a notice so that they may retrieve any data they have not yet copied for their own files.