Brenda Lilly, Ph.D.

Dr. Brenda Lilly is a Principal Investigator for the Center for Cardiovascular and Pulmonary Research at Nationwide Children's Hospital and an Associate Professor at The Ohio State University. She is a developmental biologist that studies the molecular pathways involved in blood vessel formation. Her lab examines the interactions between endothelial cells and smooth muscle cells that govern differentiation in normal and diseased states.

Gender:

• Female

Languages Spoken:

• English

Research Center:

• Center for Cardiovascular and Pulmonary Research

Areas of Interest:

• Dr. Lilly’s research focuses on understanding the regulatory pathways that control vascular development and smooth muscle differentiation. Using a 3-D culture system to grow blood vessels in vitro, her lab is investigating how smooth muscle precursors are summoned to the nascent vessel. Recruitment of smooth muscle cells is thought to be dependent upon paracrine signals emanating from the endothelial cell tube; however the mechanisms of these signaling events remain a mystery. Dr. Lilly is also working to define the transcriptional mechanisms that govern selective gene expression in smooth muscle cells. Using smooth muscle regulatory enhancers as tools, her lab directly tests the response that individual components of signaling pathways have on gene expression. Lab Web Site: http://www.nationwidechildrens.org/lilly-lab

Undergraduate School

• SUNY College at Geneseo
  Date Completed: 06/30/1986

Post Doctoral

• University of Texas, MD Anderson Cancer Center, Graduate School of Biomedical Sciences
  Date Completed: 06/30/1995

2010–present

• Associate Professor, Department of Pediatrics, The Ohio State University Medical Center, Columbus, OH

2010–present

• Principle Investigator, Center for Cardiovascular and Pulmonary Research, The Research Institute at Nationwide Children's Hospital, Columbus, OH

2007–2010

• Associate Professor, Vascular Biology Center, Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta, GA

2002–2007

• Assistant Professor, Vascular Biology Center, Department of Obstetrics and Gynecology, Medical College of Georgia, Augusta GA

1998–2002

• Postdoctoral Research, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT

1995–1998

• Postdoctoral Research, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX

1991–1995

• PhD Dissertation: The molecular and genetic characterization of the MADS box transcription factor Drosophila MEF2

• Wang,Qingqing; Zhao,Ning; Kennard,Simone; Lilly,Brenda. 2012. Notch2 and Notch3 Function Together to Regulate Vascular Smooth Muscle Development.  PLOS ONE. Vol. 7, no. 5. (May): ee37365.
• Saha S, Wang J, Buckley B, Wang Q, Lilly B, Chernov M, Kashina A. 2012. Small molecule inhibitors of arginyltransferase regulate arginylation-dependent protein degradation, cell motility, and angiogenesis.  Biochem Pharmacol.. Vol. 83(7) Epub 2012 Jan 18., no. April: 866-73.
• Pajaniappan,M; Glober,NK; Kennard,S; Liu,H; Zhao,N; Lilly,B. 2011. Endothelial cells downregulate apolipoprotein D expression in mural cells through paracrine secretion and Notch signaling.  AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY. Vol. 301, no. 3. (September): H784-H793.
• Pajaniappan, M., Glober, N.K., Kennard, S., Liu, H., Zhao, N. and Lilly, B. (2011) Endothelial cells downregulate apolipoprotein D expression in mural cells through paracrine secretion and Notch signaling. Am J Physiol Heart Circ Physiol, 301, H784- 793.
• Liu,H; Zhang,W; Kennard,S; Caldwell,R,B; Lilly,B. 2010. Notch3 Is Critical for Proper Angiogenesis and Mural Cell Investment.  CIRCULATION RESEARCH. Vol. 107, no. 7. (October): 860-?.
• Lilly B, Clark KA, Yoshigi M, Pronovost S, Wu ML, Periasamy M, Chi M, Paul RJ, Yet SF, Beckerle MC. 2010. Loss of the serum response factor cofactor, cysteine-rich protein 1, attenuates neointima formation in the mouse.  Arterioscler Thromb Vasc Biol.. Vol. 30(4), no. April: 694-701.
• Lilly, B., Clark K.A., Yoshigi, M., Pronovost, S., Wu, M.L., Periasamy, M., Chi, M., Paul, R.J., Yet, S.F., and Beckerle, M.C. 2010. Loss of the SRF co-factor, cysteine-rich protein 1, attenuates neointima formation in the mouse. Arterioscler Thromb Vasc Biol. Apr;30(4):694-701.
• Liu H, Zhang, W., Kennard, S., Caldwell, RB, Lilly, B. 2010. Notch3 regulates angiogenesis and mural cell investment. Circ. Res. 107:860-870 PubMed ID: 20689064
• Liu H, Zhang, W., Kennard, S., Caldwell, RB, Lilly, B. 2010. Notch3 regulates angiogenesis and mural cell investment. Circ Res. Aug 5. [Epub ahead of print] PubMed ID: 20689064
• Zhang,W; Rojas,M; Lilly,B; Tsai,NT; Lemtalsi,T; Liou,GI; Caldwell,RW; Caldwell,RB. 2009. NAD(P)H Oxidase-Dependent Regulation of CCL2 Production during Retinal Inflammation.  INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. Vol. 50, no. 6. (June): 3033-3040.
• Liu,H; Kennard,S; Lilly,B. 2009. NOTCH3 Expression Is Induced in Mural Cells Through an Autoregulatory Loop That Requires Endothelial-Expressed JAGGED1.  CIRCULATION RESEARCH. Vol. 104, no. 4. (February): 466-U97.
• Zhang, W., Rojas, M., Lilly, B., Tsai, N.T., Lemtalsi, T., Liou, G., Caldwell, R. W., Caldwell, R. B. 2009. NAD(P)H oxidase regulates CCL2 production during retinal inflammation. Invest Ophthalmol Vis Sci. Jun;50(6):3033-40.
  
• Lilly,B; Kennard,S. 2009. Differential gene expression in a coculture model of angiogenesis reveals modulation of select pathways and a role for Notch signaling.  PHYSIOLOGICAL GENOMICS. Vol. 36, no. 2. (January): 69-78.
• Lilly, B., and Kennard, S. 2009. Differential gene expression in a coculture model of angiogenesis reveals modulation of select pathways and a role for Notch signaling. Physiol Genomics, 36: 69-78.
• Liu, H., Kennard, S., Lilly, B. 2009. NOTCH3 expression is induced in mural cells through an autoregulatory loop that requires endothelial-expressed JAGGED1. Circ. Res., 104: 466-475.
• Liu,H; Chen,B; Lilly,B. 2008. Fibroblasts potentiate blood vessel formation partially through secreted factor TIMP-1.  ANGIOGENESIS. Vol. 11, no. 3. (September): 223-234.
• Snider,Paige; Fix,Jana,L; Rogers,Rhonda; Peabody-Dowling,Goldie; Ingram,David; Lilly,Brenda; Conway,Simon,J. 2008. Generation and characterization of Csrp1 enhancer-driven tissue-restricted Cre-recombinase mice.  GENESIS. Vol. 46, no. 3. (March): 167-176.
• Kennard,S; Liu,H; Lilly,B. 2008. Transforming growth factor-beta(TGF-beta 1) down-regulates Notch3 in fibroblasts to promote smooth muscle gene expression.  JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 283, no. 3. (January): 1324-1333.
• Snider, P., Fix, J.L., Rogers, R., Ingram, D., Lilly, B. and Conway, S.J. 2008. Characterization of CRP1Cre mice using both ROSA26R reporter and ROSAEGFP-DT transgenic mice. Genesis, Mar 7;46(3):167-176.
  
• Liu, H., Chen, B., and Lilly, B. 2008. Fibroblasts enhance blood vessel formation partially through secreted factor TIMP-1. Angiogenesis, 11; 223-234.
• Kennard, S., Liu, H., and Lilly, B. 2008. TGF-ß1 downregulates Notch3 in fibroblasts to promote smooth muscle gene expression. J Biol Chem. Jan 18;283(3):1324-33.
  
• Lindsley,Andrew; Snider,Paige; Zhou,Hongming; Rogers,Rhonda; Wang,Jian; Olaopa,Michael; Kruzynska-Frejtag,Agnieszka; Koushik,Shrinagesh,V; Lilly,Brenda; Burch,John,BE; Firulli,Anthony,B; Conway,Simon,J. 2007. Identification and characterization of a novel Schwann and outflow tract endocardial cushion lineage-restricted periostin enhancer.  DEVELOPMENTAL BIOLOGY. Vol. 307, no. 2. (July): 340-355.
• Linsley, A., Snider, P., Zhou, H., Olaopa, M., Koushik, S.V., Lilly, B., Burch, J.B., Firulli, A.B., Conway, S.J. 2007. A YY1-containing upstream 37bp enhancer is critical for expression of the periostin adhesion factor in Schwann and outflow tract endocardial cushion cell lineages. Dev. Biol. Jul 15;307(2):340-55.
  
• Taylor, C.J., Motamed, K., and Lilly, B. 2006. Protein kinase C and downstream signaling pathways in a 3-dimensional model of phorbol ester-induced angiogenesis. Angiogenesis; 9(2): 39-51.
• Corley,KM; Taylor,CJ; Lilly,B. 2005. Hypoxia-inducible factor 1 alpha modulates adhesion, migration, and FAK phosphorylation in vascular smooth muscle cells.  JOURNAL OF CELLULAR BIOCHEMISTRY. Vol. 96, no. 5. (December): 971-985.
• Corley, K.M., Taylor, C.J., and Lilly, B. 2005. Hypoxia-inducible 1? modulates adhesion, migration, and FAK phosphorylation in vascular smooth muscle cells. J Cell Biochem., 96, 971-985.
• Nawjer, I, and Lilly, B., 2005. Calcium/Calmodulin-dependent kinase IV activates Cysteine-rich Protein 1 through adjacent CRE and CArG elements. Am J Physiol Cell Physiol 289, C785-93.
• Lilly,B; Olson,EN; Beckerle,MC. 2001. Identification of a CArG box-dependent enhancer within the cysteine-rich protein 1 gene that directs expression in arterial but not venous or visceral smooth muscle cells.  DEVELOPMENTAL BIOLOGY. Vol. 240, no. 2. (December): 531-547.
• Lilly, B., Olson, E.N., and Beckerle, M.C. 2001. Identification of a CArG box-dependent enhancer within the CRP1 gene that directs expression in arterial but not venous or visceral smooth muscle cells. Dev. Biol. 240, 531-547.
• Stronach,BE; Renfranz,PJ; Lilly,B; Beckerle,MC. 1999. Muscle LIM proteins are associated with muscle sarcomeres and require dMEF2 for their expression during Drosophila myogenesis.  MOLECULAR BIOLOGY OF THE CELL. Vol. 10, no. 7. (July): 2329-2342.
• Lilly, B., O’keefe, D.D., Thomas, J.B., and Botas, J. 1999. The LIM homeodomain protein dLim1 defines a subclass of neurons within the embryonic ventral nerve cord of Drosophila. Mech. Dev. 88, 195-205.
• Lilly B; Botas J. 1999. Genetic analysis and generation of a duplication that covers the 8B region of the X chromosome in Drosphila melangaster.  Dros Info Serv. Vol. 82, no. January: 105-112.
• Stronach, B.E., Renfranz, P.J., Lilly, B. and Beckerle, M.C. 1999. Muscle LIM proteins are associated with sarcomeres and require dMEF2 for their expression during Drosophila myogenesis. Mol. Biol. Cell, 10, 2329-2342.
• Lilly B; O'Keefe DD; Thomas JB; Botas J. 1999. The LIM homeodomain protein dLim 1 defines a subclass of neurons within the embryonic ventral nerve cord of Drosphila.  Mech Dev. Vol. 88, no. January: 195-205.
• Lilly, B., and Botas, J. 1999.  Genetic analysis and generation of a duplication that covers the 8B region of the X chromosome in Drosophila melanogaster.  Dros. Info. Serv. 82, 105-112.
• Damm,C; Wolk,A; Buttgereit,D; Loher,K; Wagner,E; Lilly,B; Olson,EN; Hasenpusch-Theil,K; Renkawitz-Pohl,R. 1998. Independent regulatory elements in the upstream region of the Drosophila beta 3 tubulin gene (beta Tub60D) guide expression in the dorsal vessel and the somatic muscles.  DEVELOPMENTAL BIOLOGY. Vol. 199, no. 1. (July): 138-149.
• Damm, C., Wolk, A., Buttgereit, D., Loher, K., Wagner, E., Lilly, B., Olson, E.N., Hasenpusch-Theil, K. and Renkawitz-Pohl, R.  1998.  Independent regulatory elements in the upstream region of the Drosophila b3 tubulin gene guide expression in the dorsal vessel and the somatic muscles. Dev. Biol. 199, 138-149.
  
• Harris,A,N; RUIZLOZANO,P; Chen,Y,F; Sionit,P; Yu,Y,T; Lilly,B; Olson,E,N; Chien,K,R. 1997. A novel A/T-rich element mediates ANF gene expression during cardiac myocyte hypertrophy.  JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY. Vol. 29, no. 2. (February): 515-525.
• Harris, A.N., Ruiz-Lozano, P., Chen, Y.F., Sionit, P., Yu, Y.T., Lilly, B., Olson, E.N. and Chien, K.R. 1997. A novel A/T-rich element mediates ANF gene expression during cardiac myocyte hypertrophy.  J. Mol. Cell. Cardiol. 29(2), 515-525.
• Lilly,B; Zhao,B; Ranganayakulu,G; Paterson,B,M; Schulz,R,A; Olson,E,N. 1995. REQUIREMENT OF MADS DOMAIN TRANSCRIPTION FACTOR D-MEF2 FOR MUSCLE FORMATION IN DROSOPHILA.  SCIENCE. Vol. 267, no. 5198. (February): 688-693.
• Lilly, B., Zhao, B., Ranganayakulu, G., Paterson, B.M., Schulz, R. A. and Olson, E.N. 1995. The MADS domain transcription factor D-MEF2 is essential for muscle formation during Drosophila embryogenesis.  Science  267, 688-693.
• Lilly,B; GALEWSKY,S; Firulli,A,B; Schulz,R,A; Olson,E,N. 1994. D-MEF2 - A MADS BOX TRANSCRIPTION FACTOR EXPRESSED IN DIFFERENTIATING MESODERM AND MUSCLE-CELL LINEAGES DURING DROSOPHILA EMBRYOGENESIS.  PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. Vol. 91, no. 12. (June): 5662-5666.
• Cserjesi,P; Lilly,B; Hinkley,C; Perry,M; Olson,E,N. 1994. HOMEODOMAIN PROTEIN MHOX AND MADS PROTEIN MYOCYTE ENHANCER-BINDING FACTOR-II CONVERGE ON A COMMON ELEMENT IN THE MUSCLE CREATINE-KINASE ENHANCER.  JOURNAL OF BIOLOGICAL CHEMISTRY. Vol. 269, no. 24. (June): 16740-16745.
• Kuratani,S; Martin,JF; Wawersik,S; Lilly,B; Eichele,G; Olson,EN. 1994. THE EXPRESSION PATTERN OF THE CHICK HOMEOBOX GENE GMHOX SUGGESTS A ROLE IN PATTERNING OF THE LIMBS AND FACE AND IN COMPARTMENTALIZATION OF SOMITES.  DEVELOPMENTAL BIOLOGY. Vol. 161, no. 2. (February): 357-369.
• Cserjesi, P., Lilly, B., Hinkley, C., Perry, M. and Olson, E.N. 1994. Homeodomain protein MHox and the MADS protein myocyte enhancer-binding factor-2 converge on a common element in the muscle creatine kinase enhancer.  J. Biol. Chem. 269, 16740-16745.
• Kuratani, S., Martin, J.F., Wawersik, S., Lilly, B., Eichele, G. and Olson, E.N. 1994. The expression pattern of the chick homeobox gene gMHox suggests a role in patterning of the limbs and face and in compartmentalization of somites.  Dev. Biol. 161, 357-369.
• Lilly, B., Galewsky, S., Firulli, A.B., Schulz, R.A. and Olson, E.N. 1994.  D-MEF2: A MADS box transcription factor expressed in differentiating mesoderm and muscle cell lineages during Drosophila embryogenesis.  Proc. Natl. Acad. Sci. USA  91, 5662-5666.
  
• Cserjesi,P; Lilly,B; Bryson,L; Wang,Y,Q; Sassoon,D,A; Olson,E,N. 1992. MHOX - A MESODERMALLY RESTRICTED HOMEODOMAIN PROTEIN THAT BINDS AN ESSENTIAL SITE IN THE MUSCLE CREATINE-KINASE ENHANCER.  DEVELOPMENT. Vol. 115, no. 4. (August): 1087-1101.
• Cserjesi, P., Lilly, B., Bryson, L., Wang, Y., Sassoon, D.A. and Olson, E.N. 1992. MHox: a mesodermally-restricted homeodomain protein that binds an essential site in the muscle creatine kinase enhancer. Development  115, 1087-1101.
• Frazier ML, Lilly BJ, Wu EF, Ota T, Hewett-Emmett D. 1990. Carbonic anhydrase II gene expression in cell lines from human pancreatic adenocarcinoma.  Pancreas. Vol. 5, no. 5. (September): 507-514.
• Frazier, M.L., Lilly, B., Wu, E.F., Ota, T. and Hewett-Emmett, D. 1990.  Carbonic anhydrase II gene expression in cell lines from human pancreatic adenocarcinoma.  Pancreas  5, 507-514.
• Najwer I, Lilly B. Ca2+/calmodulin-dependent protein kinase IV activates cysteine-rich protein 1 through adjacent CRE and CArG elements.  Am J Physiol Cell Physiol..
• Zhao N, Liu H, Lilly B. Reciprocal regulation of SYNDECAN-2 and notch signaling in vascular smooth muscle cells.  J Biol Chem. 2012 Mar 21. [Epub ahead of print].
• Taylor CJ, Motamed K, Lilly B. 0. Protein kinase C and downstream signaling pathways in a three-dimensional model of phorbol ester-induced angiogenesis.  Angiogenesis.